Rapid Plasmid Isolations Using Efficient Sedimentation Program Overspeed Control in␣ the OptimaTM XL Ultracentrifuge from Beckman
نویسنده
چکیده
BECKMAN stressing. ESP allows rotor speed to be reduced automatically before precipitation can occur. Since the time required for gradient formation decreases with faster rotor speeds, this approach results in more rapid separations. In general, for fixed angle rotors, rotor speed is limited by the need to avoid precipitation of the gradient salt at the bottom of the tube, where the concentration is greatest as the gradient develops. By simulating the time-dependent changes in salt concentration throughout the gradient, ESP allows one to design protocols in which the rotor speed is maximized, without gradient salt precipitation.
منابع مشابه
DS-819: Overview of Sedimentation Velocity for the OptimaTM XL-A Analytical Ultracentrifuge
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